Tricarbonyl Complexes with Tridentate Chelators for Myocardium Imaging

ABSTRACT

Chelators of the formulae (I), (II) and (III) 
     
       
         
         
             
             
         
       
     
     and tricarbonyl complexes of radioisotopes of Tc and Re bound to them, for use in myocardial imaging.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. patent application Ser. No. 12/515,325, filed May 18, 2009, which claims the priority of PCT Application PCT/EP2007/010216, filed Nov. 23, 2007, which claims the priority of GB Application No. 0623482.7, which was filed Nov. 24, 2006.

FIELD OF THE INVENTION

The present invention relates to the field of radiopharmaceuticals for diagnostic imaging, particularly of the myocardium, and provides cationic and lipophilic organometallic complexes of radioisotopes of Tc and Re, and preferably ^(99m)Tc(I) organometallic complexes, which exhibit marked accumulation in the heart. These complexes contain a fac-[^(99m)Tc(CO)₃]⁺ core and are anchored on novel pyrazolyl-containing tridentate chelators having at least one ether group, which are also within the scope of the invention. As these complexes accumulate in the myocardium, they are well suited for cardiovascular imaging.

BACKGROUND OF THE INVENTION

In the field of cardiology, radioactive probes can provide information on the physiology and pathophysiology of the heart function, e.g. by myocardial perfusion, metabolism or innervation. Two ^(99m)Tc radiopharmaceuticals, Sestamibi and Tetrafosmin, are commercially available and approved for myocardial perfusion studies. These radiopharmaceuticals, which consist respectively of lipophilic Tc(I) and Tc(V) cationic complexes, suffer from low heart/liver and heart/lung uptake ratios, and so are not entirely satisfactory for myocardial perfusion studies. We have therefore been seeking alternative and better performing ^(99m)Tc complexes for myocardial imaging, profiting from the recently-introduced so-called organometallic labelling approach. This approach is based on complexes containing the fac-[^(99m)Tc(CO)₃]⁺ core and is assuming a growing importance in the development of ^(99m)Tc radioactive drugs for diagnostic medical applications.

We have now found that the combination of this organometallic core with appropriate ligands (appropriate in terms of charge, denticity or topology) provides cationic and lipophilic ^(99m)Tc complexes with the biological properties required for myocardial imaging.

DETAILED DESCRIPTION OF THE INVENTION

According to one aspect of this invention there are provided tridentate chelators of the type tris(pyrazolyl)methane (R₁C(pz*)₃) and bis(pyrazolyl)amine ((pz*)₂CH(CH₂)_(n)NHR₁ or (pz*)(CH₂)_(n)NR1(CH₂)_(n)(pz*)) of formulae (I), (II) and (III) respectively:

wherein each of R₁, R₂, R₃ and R₄ is independently hydrogen; a linear or branched, saturated or unsaturated C₁ to C₉ alkyl; a saturated or unsaturated carbocyclic group; a saturated or unsaturated heterocyclic or heteroaliphatic group with one or more atoms selected from O, N and S, wherein said carbocyclics, heterocyclics and heteroaliphatics are optionally substituted by one or more linear or branched, saturated or unsaturated C₁ to C₉ alkyls; an ether group —R^(X)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), wherein R^(X) and R^(Y) are independently linear or branched, saturated or unsaturated C₁ to C₉ alkyl, or saturated or unsaturated carbocyclics, any of which alkyl and/or carbocyclic groups may be substituted or unsubstituted, with the proviso that at least one of the substituents R₁, R₂, R₃ and R₄ is a linear or macrocyclic ether group of the type —R^(X)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), respectively, with the further proviso that when the tridentate chelator is of the type of formula (1), when R₁ is —R^(X)—O—R^(Y) wherein R^(Y) is substituted, and R₂, R₃, and R₄ are each H, R^(Y) is not substituted apically by a further tris(pyrazolyl)methane moiety; and when R₁ is —R^(X)—O—R^(Y) and R₂, R₃ and R₄ are each H, R₁ cannot be —CH₂—O—CH₂— (p-^(t)Bu-C₆H₄).

Preferably, the alkyl groups are each independently methyl, ethyl, n-propyl, propyl, n-butyl, t-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, or n-nonyl.

More preferably, in each case, the alkyl groups are C₁ to C₃ alkyl groups, i.e. methyl, ethyl, n-propyl, and i-propyl.

If the alkyl and/or aryl groups are substituted, they may be substituted by one or more groups selected from alkyl, aryl, alkaryl, aralkyl, hydroxy, halogen, amino, nitro, alkoxy or carboxylic acid groups.

Preferably, if neither of R₂ and R₄ is an ether group, they are the same.

According to another aspect of the invention, R₂ and R₄ are both ether groups (linear or macrocyclic and preferably the same), in which case R₃ is preferably not an ether, but may be any of the other substituents listed above. In that case R₁ can be any of the substituents listed above, including an ether group that may be a different ether group from R₂ and R₄.

According to another aspect of the invention, R₃ is a linear or macrocyclic ether group. R₂ and R₄ are the same and can be any of the substituents listed above, but preferably not ether groups. In that case R₁ can also be any of the substituents listed above, including an ether group that may be the same or different from R₃.

The carbocyclic groups are preferably 5- or 6-membered rings, more preferably cyclopentyl, cyclohexyl or phenyl groups.

The heterocyclic groups are preferably 5- to 15-membered rings, more preferably containing oxygen, nitrogen, sulphur, or any combination thereof, and are most preferably macrocyclic ethers of the type [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5, provided that x+y+z=5-15).

The heteroaliphatic groups preferably contain oxygen, nitrogen or sulphur atoms, or any combination thereof, and most preferably are ether groups of the type —R^(X)—β—R^(Y), wherein R^(X) and R^(Y) are as defined above.

As can be seen from the above formulae, the linear or macrocyclic ether groups may be attached to the 3-, 4- and/or 5-positions of the pyrazolyl rings. Also, in formula (I) the ether group may be attached to the central carbon atom, in formula (II) to the terminal amine, and in formula (III) the ether group may be attached to the central nitrogen atom. In one embodiment, at least R₁ is an ether group in the respective formulae.

Preferably, the linear ethers are alkyl ethers, but they may also be aryl ethers or alkyl ethers which also contain an aryl substituent. Preferred ethers include those of the general formula (CH₂)_(n)OR, wherein n=1, 2 or 3, and R=methyl, ethyl or n-propyl.

The macrocyclic ethers are preferably crown ethers of the general formula [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5).

The ligands shown in formulae (I), (II) and (III) above can stabilize cationic tricarbonyl complexes of the type fac-[M(CO)₃(NNN)]⁺ (Re, ^(99m)Tc), wherein NNN represents the tridentate chelator of any of the formulae I-III.

According to another aspect of the invention, there is provided a process for the preparation of tridentate chelators of formulae (I), (II) and (III), comprising contacting a compound of the formula

with a compound selected from a trihalomethane, a compound of the formula

or a compound of the formula Hal-(CH₂)_(n)—NR¹—(CH₂)_(n)-Hal (wherein Hal=a halogen), and reacting a subsequent product with an alkyl halide, aryl halide, or a halide substituted ether compound, wherein each of R₁, R₂, R₃ and R₄ is independently hydrogen; a linear or branched, saturated or unsaturated C₁ to C₉ alkyl; a saturated or unsaturated carbocyclic group; a group of formula CO₂R₅ or (CH₂)_(n)OH wherein R₅ is independently hydrogen or a linear or branched, saturated or unsaturated C₁ to C₉ alkyl and n=1-6; a saturated or unsaturated heterocyclic or heteroaliphatic group with one or more atoms selected from O, N and S, wherein said carbocyclics, heterocyclics and heteroaliphatics are optionally substituted by one or more linear or branched, saturated or unsaturated C₁ to C₉ alkyls; an ether group —R^(X)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), wherein R^(X) and R^(Y) are independently linear or branched, saturated or unsaturated C₁ to C₉ alkyl, or saturated or unsaturated carbocyclics, any of which alkyl and/or carbocyclic groups may be substituted or unsubstituted, with the proviso that at least one of the substituents R₁, R₂, R₃ and R₄ is a linear or macrocyclic ether group of the type —R^(X)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), respectively, with the further proviso that when the tridentate chelator is of the type of formula (I), when R₁ is —R^(X)—O—R^(Y) wherein R^(Y) is substituted, and R₂, R₃, and R₄ are each H, R^(Y) is not substituted apically by a further tris(pyrazolyl)methane moiety; and when R₁ is —R^(X)—O—R^(Y) and R₂, R₃ and R₄ are each H, R₁ cannot be —CH₂—O—CH₂— (p-^(t)Bu-C₆H₄).

According to another aspect of the invention, tricarbonyl complexes of radioisotopes of Tc or Re bound to chelators of any of formulae (I), (II), or (III) above are also provided.

The radioisotope is preferably ^(99m)Tc. The ^(99m)Tc complexes can accumulate in the heart, therefore being useful in radiopharmaceuticals for myocardial imaging.

According to a further aspect of the invention there is provided a composition comprising a tridentate chelator as described above and a mixture of sodium boranocarbonate, sodium borate and sodium carbonate. Optionally, a transfer ligand may also be incorporated into the composition. Examples of such transfer ligands are salts of a weak organic acid, i.e. an organic acid having a pKa in the range 3 to 7, with a biocompatible cation. Such weak organic acids include acetic acid, citric acid, tartaric acid, gluconic acid, glucoheptonic acid, benzoic acid, phenols or phosphonic acids. Particularly, the transfer ligands comprise tartrate, gluconate, citrate, and/or glucoheptonate salts. Preferably, these compositions are in lyophilised form in a sterile vial.

There is also provided a composition comprising a tridentate chelator of any of formulae (I), (II), or (III) and a filler material. By filler material it is meant any material which can be combined with the composition to enable the formation of tablets or pellets of the composition. Exemplary filling materials include, but are not limited to, inositol, lactose and saccharose.

If desired, this composition may also contain an anti-oxidant substance, such as but not limited to gentisic acid, ascorbic acid or methionine.

There is also provided a process for the preparation of a tricarbonyl complex of radioisotopes of Tc or Re bound to chelators of any of formulae (I), (II), or (III), comprising contacting a compound of formula [M(X)₃(CO)₃]⁺, wherein M=Tc or Re and X═H₂O, MeOH or a halogen, with a tridentate chelator in an alcoholic or aqueous solvent, at a pH of about 6 or less.

Alternatively, the compound of formula [M(X)₃(CO)₃]⁺ can be contacted with a composition comprising a tridentate chelator of any of formulae (I), (II), or (III) and a mixture of sodium boranocarbonate, sodium borate and sodium carbonate.

The pH of the reaction is preferably about 4 or less, and the alcoholic solvent is preferably ethanol.

The compound of formula [M(X)₃(CO)₃]⁺ can be prepared by adding ^(99m)Tc-pertechnetate in saline (which is preferably obtained from a ⁹⁹Mo/^(99m)Tc generator), or a perrhenate complex, to a composition comprising sodium boranocarbonate, sodium borate and sodium carbonate. An example of such a composition is IsoLink®, which comprises sodium boranocarbonate, sodium borate, sodium carbonate and a tartrate salt.

A general mode of preparation of the tricarbonyl complex of radioisotopes of Tc or Re bound to chelators of any of formulae (I), (II), or (III), involves a pertechnetate or perrhenate being added to a solution containing the mixture comprising sodium boranocarbonate, sodium borate and sodium carbonate, and this is then heated to yield the tricarbonyl precursor [M(H₂O)₃(CO)₃]⁺ (M=Tc, Re). The solution (which has a pH of about 10.5) is acidified to about pH 4, mixed with a tridentate chelator ligand and heated again to allow the reaction between the tricarbonyl complex and the ligand.

The boranocarbonate-containing composition is used to reduce the pertechnetate (or perrhenate) from oxidation state VII to I and, at the same time, to generate carbon monoxide which will coordinate and stabilize the Tc(I) in [Tc(H₂O)₃(CO)₃]⁺.

There is also provided a method of myocardial imaging comprising administering an imaging composition comprising an effective amount of a tricarbonyl complex of radioisotopes of Tc or Re bound to chelators of any of formulae (I), (II), or (III) to a patient. Preferably, the imaging composition is injected intravenously into the patient, who is either at rest or has undergone exercise prior to administration, where an assessment of myocardial bloodflow is necessary. SPECT or planar scintigraphy is then performed after a relevant period post injection and the results of the biodistribution of the radioactive compound in the myocardium are evaluated.

The inclusion of at least one ether substituent in the tridentate chelator compounds is important. Lipophilic and cationic ^(99m)Tc complexes can cross the membranes of cardiac cells by free diffusion, and it is believed that their accumulation in the heart originates from negative mitochondrial membrane potentials. The ether substituents tune the lipophilicity of the complexes to improve both heart uptake and excretion kinetics, especially clearance from the liver, as the ether groups can increase the likelihood of the complex being metabolised by the liver. Such behaviour will improve the target-to-background ratios.

Some synthetic procedures for chelators of formulae (I) to (III) and for the respective organometallic complexes with rhenium and technetium are given in schemes 1 and 2 respectively.

Some detailed but non-limiting examples are given below, showing how some of the chelators and their respective complexes with the fac-[M(CO)₃]⁺ (where M=^(99m)Tc or Re) moieties may be synthesised, and demonstrating the affinity of such complexes for the myocardium.

Although ^(99m)Tc is the preferred radionuclide in the invention, it will be understood by those skilled in the art that other radionuclides may be used.

BRIEF DESCRIPTION OF THE FIGURES

In the examples reference is made to the following figures:

FIG. 1: Synthesis of representative examples of chelators of formula I having ether groups at the central carbon atom.

FIG. 2: Synthesis of representative examples of chelators of formula I bearing ether groups at 4-position of pyrazole.

FIG. 3: Synthesis of a representative example of chelators of formula I having an ether group at the 3- and 5-positions of the pyrazolyl ring.

FIG. 4: ORTEP drawing of the cation of compound 8 ([{HC[3,5-(CH₃OCH₂)₂pz]₃}₂Na]+).

FIG. 5: Synthesis of a representative example of chelators of formula I having ether groups in the 3-, 4- and 5-positions of the pyrazolyl ring.

FIG. 6: Synthesis of a representative example of chelators of formula II having ether groups at the primary amine.

FIG. 7: Synthesis of a representative example of chelators of formula III having ether groups at the secondary amine and/or at the 3- and 5-position of the pyrazolyl rings.

FIG. 8: Synthesis of Re complexes with a representative example of chelators of formula I.

FIG. 9: ¹H NMR spectrum of a rhenium complex with a chelator of formula I having an ether group at the central carbon atom.

FIG. 10: ¹H NMR spectrum in CDCl₃ of a rhenium complex with a chelator of formula I having ether groups at the 3- and 5-positions of the pyrazolyl rings.

FIG. 11: Molecular structure of a Re(I) tricarbonyl complex with one of the chelators of class I.

FIG. 12: Synthesis of ^(99m)Tc(I) tricarbonyl complexes with chelators of formulae I and II.

FIG. 13: HPLC chromatogram of a ^(99m)Tc(I) tricarbonyl complex with a chelator of formula I and comparison with its Re congener.

EXAMPLES Instruments

All chemicals and solvents were of reagent grade and were used without purification unless stated otherwise. ¹H and ¹¹C NMR spectra were recorded on a Varian Unity 300 MHz spectrometer; ¹H and ¹¹C chemical shifts (ppm) were referenced with the residual solvent resonances relative to tetramethylsilane. IR spectra were recorded on a Perkin-Elmer 577 spectrometer as KBr pellets. C, H and N analyses were performed on an EA 110 CE Instruments automatic analyser. Na[^(99m)TcO₄] was eluted from a Mallinckrodt Med. Inc. generator, using 0.9% saline. HPLC analysis of the complexes was performed on a Perkin-Elmer LC pump 200 coupled to a LC 290 tunable UV/Vis detector and to a Berthold LB-507A radiometric detector. Separations were achieved on a Nucleosil column (10 μm, 250 mm×4 mm), using a flow rate of 1 mL/min; UV detection, 254 nm.

Unless otherwise stated, reactions were carried out at normal pressure and under a nitrogen atmosphere. Work-ups were carried out in air.

Example 1 Production of Chelators of Formula I Having an Ether Group at the Central Carbon Atom

The synthesis of this type of chelators was accomplished via deprotonation of 2,2,2-tris(pyrazolyl)ethanol followed by its reaction with adequate alkyl iodides (FIG. 1).

Example 1a O-methyl-1,1,1-tris(pyrazolyl)ethanol: CH₃OCH₂C(pz)₃ (compound 1)

To a stirred suspension of NaH (34 mg, 1.42 mmol) in THF (10 mL) was added, at room temperature (20-25° C.), 2,2,2-tris(pyrazolyl)ethanol (300 mg, 1.23 mmol) dissolved in the same solvent (10 mL), and the mixture was stirred for 1 h. After cooling to 0° C., a solution of methyl iodide (873 mg, 6.15 mmol) in THF (5 mL) was added dropwise. The resulting mixture was warmed to room temperature (20-25° C.) and stirred for about 16 h. After evaporation of THF under vacuum, the residue was extracted twice with 10 mL of diethyl ether. Following washing of the ether extracts with distilled water, compound 1 was recovered in the form of a colourless oil, after drying the organic phase under vacuum. Yield: 97% (307 mg, 1.19 mmol).

¹H NMR (CDCl₃, δ ppm): 7.64 (3H, d, H-5 (pz)), 7.34 (d, 3H, H-3 (pz)), 6.32 (dd, 3H, H-4 (pz)), 5.03 (s, 2H, CH ₂), 3.38 (s, 3H, CH ₃O). ¹³C NMR (CDCl₃, δ ppm): 141.3 (C-3 (pz)), 130.7 (C-5 (pz)), 106.5 (C-4 (pz)), 89.5 (C-(pz)₃), 75.5 (CH₂), 59.9 (CH₃O).

Example 1b O-ethyl-1,1,1-tris(pyrazolyl)ethanol: CH₃CH₂OCH₂C(pz)₃ (compound 2)

Compound 2 is a colourless oil with tendency to solidify on standing, which was obtained as above described for compound 1, starting from 305 mg (1.25 mmol) of 2,2,2-tris(pyrazolyl)ethanol. Yield: 66% (225 mg, 0.83 mmol).

¹H NMR (CDCl₃, δ ppm): 7.63 (3H, d, H-5 (pz)), 7.40 (d, 3H, H-3 (pz)), 6.31 (dd, 3H, 3H, H-4 (pz)), 5.05 (s, 2H, CH ₂), 3.50 (q, 2H, OCH₂CH₃), 1.09 (tr, 3H, OCH₂CH ₃). ¹³C NMR (CDCl₃, δ ppm): 141.2 (C-3 (pz)), 130.9 (C-5 (pz)), 106.4 (C-4 (pz)), 89.7 (C-(pz)₃), 73.5 (CH₂), 67.9 (CH₂), 15.0 (CH₃).

Example 1c O-n-propyl-1,1,1-tris(pyrazolyl)ethanol: CH₃(CH₂)₂OCH₂C(pz)₃ (compound 3)

Compound 3 is a colourless oil which was obtained as above described for compound 1, starting from 350 mg (1.43 mmol) of 2,2,2-tris(pyrazolyl)ethanol. Yield: 52% (212 mg, 0.74 mmol).

¹H NMR (CDCl₃, δ ppm): 7.63 (3H, d, H-5 (pz)), 7.40 (d, 3H, H-3 (pz)), 6.31 (dd, 3H, H-4 (pz)), 5.05 (s, 2H, CH ₂), 3.40 (tr, 2H, OCH ₂), 1.47 (m, 2H, CH ₂), 0.77 (tr, CH ₃).

Example 2 Production of Chelators of Formula I Having an Ether Group at the 4-Position of the Pyrazolyl Ring Example 2a tris[3,5-Me₂-4-(2-methoxyethyl)pyrazolyl]methane, HC(3,5-Me₂-4-CH₃OCH₂CH₂pz)₃ (compound 4) (FIG. 2) Ethyl 2-(3,5-dimethyl-1H-pyrazol-4-yl)acetate

To a solution of ethyl 3-acetyl-4-oxopentanoate (synthesized as described D. P. Shrout and D. A. Lightner, Synth. Commun. 20 (1990), 2075) (9.676 g, 51.96 mmol) in ethanol, at 0° C., was added slowly a solution of hydrazine hydrate (2.990 g, 59.75 g) in absolute ethanol. The reaction mixture was warmed to room temperature and stirred for 2 hours. After removing ethanol under vacuum, dichloromethane and water were added to the crude product and the organic layer was separated. The aqueous layer was extracted further with dichloromethane and the combined organic extracts were washed with water. After drying over MgSO₄ and evaporation of the solvent under reduced pressure, the pyrazole was isolated as a yellow oil. Yield: 72% (6.777 g, 37.2 mmol).

¹H NMR (300 MHz, CDCl₃, δ (ppm)): 4.09 (2H, q, 7 Hz, CH₂), 3.32 (2H, s, CH₂), 2.21 (6H, s, CH₃), 1.22 (3H, t, 7 Hz, CH₃). ¹³C NMR (75.37 MHz, CDCl₃, δ (ppm)): 171.5 (COOEt), 143.0 (C-pz), 108.6 (C-pz), 60.7 (CH₂), 29.4 (CH₂), 14.2 (CH₃), 10.9 (CH₃).

3,5-dimethyl-4-ethylacetate-1-tritylpyrazole

To a solution of 3,5-dimethyl-4-ethyl-acetatepyrazole (1.939 g, 10.6 mmol) in dry THF was added sodium hydride (556 mg, 60% in mineral oil, 13.8 mmol) and the mixture was stirred at room temperature for 1 hour. Triphenylmethyl chloride (2.955 g, 10.6 mmol) was then added, and the reaction run for 3 days at room temperature. After this time, the solvent was evaporated and the solid was stirred in water, filtered and washed with water and diethyl ether. After drying under vacuum the pyrazole was obtained as a solid. Yield: 46% (2.047 g, 4.82 mmol).

¹H NMR (300 MHz, CDCl₃, δ (ppm)): 7.30-7.00 (15H, m, Ph), 4.09 (2H, q, 7.2 Hz, CH₂), 3.29 (2H, s, CH₂), 2.15 (3H, s, CH₃), 1.42 (3H, s, CH₃), 1.20 (3H, t, 7 Hz, CH₃). ¹³C NMR (75.37 MHz, CDCl₃, δ (ppm)): 171.5 (COOEt), 144.7 (C-pz), 141.1 (Ph), 139.6 (C-pz), 130.3, 127.3, 127.1 (Ph), 111.6 (C-pz), 60.6 (CH₂), 30.0 (CH₂), 14.2 (CH₃), 13.0 (CH₃), 12.4 (CH₃).

3,5-dimethyl-4-hydroxyethyl-1-tritylpyrazole

To a solution of 3,5-dimethyl-4-ethylacetate-1-tritylpyrazole (2.442 g, 5.75 mmol) in dry THF, at 0° C., LiAlH₄ in ether (1M, 11.5 mmol) was added dropwise. After stirring at room temperature for about 16 h, the reaction was quenched by addition of water (1.4 ml) and NaOH (10%, 0.5 ml). The suspension was filtered off and the supernatant was extracted with dichloromethane. The organic phase was dried over MgSO₄ and vacuum dried, yielding the title compound as a yellow solid. Yield: 90% (1.980 g, 5.176 mmol).

¹H NMR (300 MHz, CDCl₃, δ (ppm)): 7.30-7.00 (15H, m, Ph), 3.60 (2H, t, 6.8 Hz, CH₂), 2.57 (2H, t, 6.8 Hz, CH₂), 2.15 (3H, s, CH₃), 1.41 (3H, s, CH₃).

3,5-dimethyl-4-ethyl methyl ether-1-tritylpyrazole

To a solution of 3,5-dimethyl-4-hydroxyethyl-1-tritylpyrazole (1.910 g, 4.99 mmol) in dry THF was added a portion of NaH (260 mg, 60% in mineral oil, 6.49 mmol), and the mixture was stirred at room temperature for about 16 h. Methyl iodide (1.56 mL, 24.95 mmol) was then added and the reaction stirred for 24 h at 20-25° C. After removal of THF under vacuum, the resulting residue was extracted with dichloromethane, followed by washing with water. Evaporation of dichloromethane under vacuum yielded the title compound as a yellow solid. Yield: 93% (1.834 g, 4.63 mmol).

¹H NMR (300 MHz, CDCl₃, δ (ppm)): 7.30-7.00 (15H, m, Ph), 3.32 (2H+3H, CH₂+OCH₃), 2.56 (2H, t, 7.8 Hz, CH₂), 2.15 (3H, s, CH₃), 1.40 (3H, s, CH₃).

3,5-dimethyl-4-ethyl methyl etherpyrazole

A solution of 3,5-dimethyl-4-ethyl methyl ether-1-tritylpyrazole (1.832 g, 4.62 mmol) in dichloromethane/methanol (1:2, 72 mL) was treated with CF₃COOH (18 ml) and heated at 75° C. for about 16 h. After this time, the mixture was cooled to room temperature and the solvents were evaporated. The crude product was purified by silica-gel chromatography using gradient elution, from 10% n-hexane/90% ethyl acetate to 20% methanol/80% ethyl acetate. After evaporation of the solvent from the collected fractions, the isolated pyrazole contained solvated CF₃COOH. To remove the acid, the compound was redissolved in ethyl acetate and washed successively with a saturated solution of NaHCO₃ and with water. After drying the organic phase over MgSO₄ and evaporation of the solvent under vacuum, the title compound was isolated as a slightly yellow oil. Yield: 43% (305 mg, 1.98 mmol).

¹H NMR (300 MHz, CDCl₃, δ (ppm)): 3.36 (2H, t, 7.2 Hz, CH₂), 3.32 (3H, s, OCH₃), 2.60 (2H, t, 7.2 Hz, CH₂), 2.19 (6H, s, CH₃). ¹³C NMR (75.37 MHz, CDCl₃, δ (ppm)): 142.5 (C-pz), 111.7 (C-pz), 72.8 (CH₂), 58.56 (OCH₃), 23.7 (CH₂), 10.8 (CH₃).

HC(3,5-Me₂-4-CH₃OCH₂CH₂pz)₃ (compound 4)

Under vigorous stirring, Na₂CO₃ (1.259 g, 1.88 mmol) was slowly added to a mixture of 3,5-dimethyl-4-ethyl methyl etherpyrazole (300 mg, 1.9 mmol) and tetrabutyl ammonium bromide (31.3 mg, 0.097 mmol) in H₂O (2.6 ml), occurring a slightly exothermic reaction. After cooling to room temperature (20-25° C.), 1.3 mL of chloroform was added and the resulting mixture was heated at reflux for 3 days. After this time, the solids formed were filtered off and 25 mL of CHCl₃ were added to the supernatant and the organic phase separated. The organic phase was washed with water, dried over MgSO₄ and the solvent was removed under vacuum. The residue obtained was purified by silica-gel chromatography (2% methanol/98% CHCl₃) to give the tris(pyrazolyl)methane as a slightly yellow oil which crystallised at room temperature. Yield: 31% (0.195 mmol, 92 mg).

¹H NMR (300 MHz, CDCl₃, δ (ppm)): 8.02 (1H, s, CH), 3.32 (6H, t, 7.5 Hz, CH₂), 3.30 (9H, s, OCH₃), 2.57 (6H, t, 7.5 Hz, CH₂), 2.13 (9H, s, CH₃), 1.89 (9H, s, CH₃). ¹³C NMR (75.37 MHz, CDCl₃, δ (ppm)): 147.8 (C-pz), 138.1 (C-pz), 114.7 (C-pz), 81.2 (CH), 72.6 (CH₂), 58.6 (OCH₃), 24.1 (CH₂), 12.3 (CH₃), 9.2 (CH₃).

Example 2b Tris(4-methoxymethylpyrazolyl)methane: HC(4-CH₃OCH₂pz)₃ (compound 5) Ethyl 1-tritylpyrazole-4-carboxylate

Ethyl pyrazole-4-carboxylate (W. Holtzer, G. Seiringer, J. Heterocyclic Chem., 1993, 30, 865) (1.7 g, 12.1 mmol) and NaH (680 mg, 60% in mineral oil, 16.94 mmol) reacted in dry DMF for 1 h. Then, trityl chloride (3.373 g, 12.1 mmol) was added, and the reaction mixture stirred for 3 days at room temperature. The title compound was recovered as described in example 2a for 3,5-dimethyl-4-ethylacetate-1-tritylpyrazole. Yield: 82% (3.79 g, 4 mmol).

¹H NMR (CDCl₃, δ ppm): 8.02 (1H, s, H-3 (pz)), 7.91 (1H, s, H-5 (pz)), 7.40-7.27 (9H, m, Ph), 7.20-7.00 (6H, m, Ph), 4.25 (2H, q, 7.2 Hz, CH ₂), 1.29 (3H, t, 7.2 Hz, CH ₃). ¹³C NMR (CDCl₃, δ ppm): 163.2 (CO), 142.3 (3/5-C(pz)), 141.1 (C-Ph)), 135.6 (C-Ph), 130.0 (C-Ph), 127.9 (C-Ph), 113.8 (4-C(pz)), 79.4 (N—CPh3), 60.2 (CH₂), 14.4 (CH₃).

1-trityl-4-hydroxymethylpyrazole

The title compound was obtained by reduction of ethyl 1-tritylpyrazole-4-carboxylate (3.785 g, 9.9 mmol) with LiAlH₄ (19.8 mmol) in dry THF (50 mL), as described in example 2a for the synthesis of 3,5-dimethyl-4-hydroxyethyl-1-tritylpyrazole. Yield: 91% (3.06 g, mmol).

¹H NMR (CDCl₃, δ ppm): 7.65 (1H, s, H-3 (pz)), 7.37 (1H, s, H-5 (pz)), 7.24-7.30 (9H, m, Ph), 7.08-7.16 (6H, m, Ph), 4.54 (2H, s, CH ₂). ¹³C NMR (CDCl₃, δ ppm): 143.1 (3/5-C(pz)), 139.1 (C-Ph), 131.3 (C-Ph), 130.1 (C-Ph), 127.7 (C-Ph), 120.0 (4-C(pz)), 56.2 (CH₂).

1-trityl-4-methoxymethylpyrazole

This compound is obtained by reacting 1-trityl-4-hydroxymethylpyrazole (555 mg; 1.63 mmol) in dry tetrahydrofuran with sodium hydride (98 mg; 2.44 mmol) and iodomethane (0.5 mL; 8.15 mmol). Yield: 93% (536 mg, 1.51 mmol).

¹H NMR (CDCl₃, δ ppm): 7.63 (1H, s; 3-H(pz)), 7.36 (1H, s, 5-H(pz)), 7.25-7.30 (9H, m, Ph), 7.08-7.15 (6H, m, Ph), 4.29 (2H, s, CH₂), 3.31 (3H, s, CH₃).

4-methoxymethylpyrazole

CF₃COOH was added to a solution of 1-trityl-4-methoxymethylpyrazole in CH₂Cl₂/MeOH (1:1). The mixture was heated at 75-80° C. overnight. After cooling to room temperature, the solvent was removed under the vacuum and the residue was applied on the top of a silica-gel column and eluted with methanol/ethyl acetate (5:95). The solvent from the collected fractions was removed under vacuum, and the residue redissolved in ethyl acetate. The title compound was recovered as a slightly yellow oil, after washing the organic phase with a saturated solution of NaHCO₃ and distilled water, followed by removal of the solvent and prolonged drying under vacuum. Yield: 38% (60 mg, 0.54 mmol).

¹H NMR (CDCl₃, δ ppm): 7.57 (2H, s, H-3/5 (pz)), 4.38 (2H, s, CH ₂), 3.34 (3H, s, CH ₃).

HC(4-CH₃OCH₂pz)₃ (compound 5)

This compound has been synthesized as described in Example 2a for compound 4, starting from 4-methoxymethylpyrazole (60 mg, 0.54 mmol). After purification by gradient HPLC (100% aqueous 0.1% CF₃COOH solution→100%.CH₃CN), using a Nucleosil column (10 μm, 250 mm×4 mm), compound 5 was recovered as a slightly yellow oil. Yield: 16% (10 mg, 0.029 mmol).

¹H NMR (CDCl₃, δ ppm): 8.24 (1H, s, CH), 7.62 (3H, s, H-3/5 (pz)), 7.54 (3H, s, H-3/5 (pz)), 4.31 (6H, s, 6.9 Hz, CH ₂) 3.32 (9H, s, OCH ₃). ¹³C NMR (CDCl₃, δ ppm): 141.7 (C-3/5(pz)), 128.7 (C-3/5(pz)), 120.1 ((C-4(pz)), 83.3 (CH), 65.1 (CH₂), 57.9 (OCH₃). FTICR-MS(+) (m/z): 346.2[M]⁺ (12).

Tris(4-ethoxymethylpyrazolyl)methane: HC(4-CH₃CH₂OCH₂pz)₃ (compound 6)

1-trityl-4-ethoxymethylpyrazole

This compound is a slightly yellow solid and has been obtained as described in 2b for 1-trityl-4-methoxymethylpyrazole, starting from 1-trityl-4-hydroxymethylpyrazole (1.250 g; 3.67 mmol), sodium hydride (221 mg; 5.51 mmol) and iodoethane (1.5 mL; 18.35 mmol), as described in example 2a for 3,5-dimethyl-4-methoxyethyl-1-tritylpyrazole. Yield: 87% (1.174 g, 3.19 mmol).

¹H NMR (CDCl₃, δ ppm): 7.64 (1H, s, 3-H(pz)), 7.35 (1H, s, 5-H(pz)), 7.26-7.29 (9H, m, Ph), 7.10-7.13 (4H, m, Ph), 4.33 (2H, s, CH₂), (2H, q, 7.2 Hz, CH₂), 1.18 (3H, t, 7.2 Hz, CH₃). ¹³C(CDCl₃, δ ppm): 143.2 (3/5-C(pz)), 139.9 (C-Ph)), 131.8 (C-Ph), 130.1 (C-Ph), 127.7 (C-Ph), 117.1 (4-C(pz)), 65.4 (CH₂)., 63.5 (CH₂), 15.2 (CH₃).

4-ethoxymethylpyrazole

1-trityl-4-ethoxymethylpyrazole (1.800 g, 4.88 mmol) was dissolved in a mixture of ethanol/acetone (15 ml/5 ml) and 30 mL of 2N HCl was added to the resulting solution. The mixture was heated at 80° C. for 2 h. After cooling to room temperature, the reaction mixture was filtered and washed with dichloromethane. The aqueous layer was basified with 2N NaOH and extracted with dichloromethane. The organic layers were combined, dried over MgSO₄ and the solvent was evaporated under vacuum yielding compound 4-ethoxymethylpyrazole as a white oil. Yield: 44% (270 mg, 2.14 mmol).

¹H NMR (CDCl₃, δ ppm): 7.57 (2H, s, H-3/5 (pz)), 4.43 (2H, s, CH₂), 3.50 (2H, q, 6.9 Hz, CH ₂), 1.20 (3H, t, 6.9 Hz, CH3). ¹³C NMR (CDCl₃, δ ppm): 133.6 (C-3/5 (pz)), 117.9 (C-4 (pz)), 65.3 (CH₂), 63.3 (CH₂), 15.1 (CH₃).

HC(4-CH₃CH₂OCH₂pz)₃ (compound 6)

This compound has been obtained as described in Example 2a for compound 4. Starting with 4-ethoxymethylpyrazole (234 mg, 1.86 mmol), and after convenient work-up, a white microcrystalline solid was obtained. Yield: 73% (176 mg, 0.453 mmol).

¹H NMR (CDCl₃, δ ppm): 8.25 (1H, s, CH), 7.60 (3H, s, H-3/5 (pz)), 7.55 (3H, s, H-3/5 (pz)), 4.32 (6H, s, 6.9 Hz, CH ₂) 3.45 (6H, q, OCH ₂), 1.15 (9H, t, CH ₃). ¹³C NMR (CDCl₃, δ ppm): 141.6 (C-3/5(pz)), 128.6 (C-3/5(pz)), 120.2 ((C-4(pz)), 83.15 (CH), 65.6 (CH₂), 63.0 (CH₂), 15.0 (CH₃). FTICR/MS(+) (m/z): 388.2 [M]⁺ (50%).

Example 3 Production of Chelators of Formula I Having Alkoxy Groups at 3-,5-Positions of the Pyrazolyl Ring Example 3a tris[3,5-(methoxymethyl)pyrazolyl]methane, HC [3,5-(CH₃OCH₂)₂pz]₃ (compound 7) (FIG. 3). Dimethyl 1-trityl-3,5-pyrazoledicarboxylate

This compound is a white microcrystalline solid that has been isolated following the methodology described in 2b for ethyl-1-tritylpyrazole-4-carboxylate, by reaction of with dimethyl-3,5-pyrazoledicarboxylate (1.411 g, 7.66 mmol) with sodium hydride (250 mg, 10.41 mmol) in dry dimethylformamide (20 mL), followed by treatment with trityl chloride (2.135 g, 7.66 mmol). Yield: 98% (3.200 g, 7.50 mmol).

¹H NMR (CDCl₃, δ ppm): 7.34 (1H, s, H-4 (pz)), 7.26 (9H, m, Ph), 7.08 (6H, m, Ph), 3.83 (3H, s, CH ₃), 3.26 (3H, s, CH ₃).

1-trityl-3,5-bis(hydroxymethyl)pyrazole

To a solution of dimethyl 1-trityl-3,5-pyrazoledicarboxylate (3.200 g, 7.50 mmol) in dry THF (50 mL) were added 30 mL of 1.0 M LiAlH₄ in Et₂O. The resulting solution was stirred for about 16 h at room temperature (20-25° C.). After this time, the reaction was quenched by the slow addition of 1 mL of distilled water, followed by addition of 1 mL of 10% NaOH and 2.4 mL of water. The solids formed were removed by filtration, and 50 mL of dichloromethane was added to the filtrate. The organic phase was separated, washed with distilled water and a white microcrystalline solid was obtained after evaporation of the solvent under vacuum. Yield: 90% (2.843 g, 6.71 mmol).

¹H NMR (CDCl₃, δ ppm): 7.28 (9H, m, Ph), 7.08 (6H, m, Ph), 6.44 (1H, s, H-4 (pz)), 4.62 (2H, d, CH ₂), 3.83 (2H, d, CH ₂), 2.10 (1H, tr, OH), 0.70 (1H, tr, OH).

1-trityl-3,5-bis(methoxymethyl)pyrazole

This compound is a white microcrystalline solid that has been obtained by reacting 1-trityl-3,5-bis(hydroxymethyl)-pyrazole (2.483 g, 6.71 mmol) with sodium hydride (670 mg; 16.8 mmol) and methyl iodide (4.2 mL; 67.5 mmol), as described above in examples 2a and 2b for congener pyrazole derivatives. Yield: 98% (2.606 g, 6.55 mmol).

¹H NMR (CDCl₃, δ ppm): 7.26 (9H, m, Ph), 7.09 (6H, m, Ph), 6.45 (1H, s, H-4 (pz)), 4.39 (2H, s, CH ₂), 3.48 (2H, s, CH ₂), 3.34 (3H, s, CH ₃), 2.97 (3H, s, CH ₃).

3,5-bis(methoxymethyl)pyrazole

This compound was obtained by deprotection of trityl-3,5-bis(hydroxymethyl)pyrazole (2.606 g, 6.54 mmol), as described in example 2b for 4-methoxymethylpyrazole. Yield: 58% (589 mg, 3.80 mmol).

¹H NMR (CDCl₃, δ ppm): 10.72 (1H, br, NH), 6.19 (1H, s, H-4 (pz)), 4.45 (4H, s, CH ₂), 3.32 (6H, s, CH ₃). ¹³C NMR (CDCl₃, δ ppm): 145.2 (C-3/5 (pz)), 103.4 (C-4 (pz)), 66.5 (CH ₂), 58.0 (CH ₃).

tris[3,5-(methoxymethyl)pyrazolyl]methane, HC[3,5-(CH₃OCH₂)₂pz]₃ (compound 7)

To a solution of 3,5-bis(methoxymethyl)pyrazole (331 mg, 2.14 mmol) and [NBu₄]Br (35 mg, 0.11 mmol) in distilled water (3 mL) was slowly added Na₂CO₃ (1.77 g, 2.64 mmol). Then, 1.5 mL of CHCl₃ was added and the mixture was heated gently under reflux for 3 days. The organic phase was separated, washed with water and dried over MgSO₄. Compound 7 was purified by silica-gel flash chromatography using methanol/dichloromethane (2:98) as eluent. Yield: 34% (116 mg, 0.24 mmol).

Anal. Calcd. for C₂₂H₃₄N₆O₆: C, 55.22; H, 7.16; N, 17.56%. Found: C, 55.89; H, 5.49; N, 17.53%.

¹H NMR (CDCl₃, δ ppm): 8.71 (1H, s, CH), 6.33 (3H, s, H-4 (pz)), 4.38 (6H, s, CH ₂), 4.30 (6H, s, CH ₂) 3.31 (9H, s, CH ₃) 3.18 (9H, s, CH ₃). ¹³C NMR (CDCl₃, δ ppm): 149.7 (C-3/5(pz)), 141.1 (C-3/5(pz)), 107.4 (C-4(pz)), 78.7 (C—H), 68.2 (CH₂), 64.6 (CH₂), 57.9, 57.8 (OCH₃). FTICR/MS(+) (m/z): 479.3 [M+H]⁺ (22%).

Example 3b Sodium salt of tris[3,5-(methoxymethyl)pyrazolyl]methane: [{HC[3,5-(CH₃OCH₂)₂pz]₃}₂Na]I (compound 8)

Sodium salt of tris[3,5-(methoxymethyl)pyrazolyl]methane, [{HC[3,5-(CH₃OCH₂)₂pz]₃}₂Na]I (compound 8)

To a solution of Nat (14 mg, 0.093 mmol) in dry THF (2 mL) was added dropwise a solution of tris[3,5-(methoxymethyl)pyrazolyl]methane (compound 7) (89 mg, 0.186 mmol) in THF. After one hour of reaction at room temperature, the formed white precipitate was separated and vacuum dried. Yield: 68% (70 mg, 0.063 mmol). Elemental analysis: Found (Calcd.) for C₄₄H₆₈IN₁₂NaO₁₂: C, 48.35 (47.74); H, 5.97 (6.19); N, 15.34 (15.18);

¹H NMR (D₂O, δ ppm): 8.55 (1H, s, CH), 6.42 (3H, s, H-4 (pz)), 4.30-4.29 (12H, s, CH ₂), 3.20 (9H, s, CH ₃) 3.02 (9H, s, CH ₃); ¹³C NMR (D₂O, δ ppm): 152.3 (C-3/5(pz)), 143.4 (C-3/5(pz)), 111.3 (C-4(pz)), 79.3 (C—H), 69.1 (CH₂), 65.7 (CH₂), 59.7 (OCH₃)., 59.6 (OCH₃). Monocrystals suitable for X-ray structural analysis were obtained by recrystallization of compound 8 from a mixture of acetone/n-hexane.

Example 3c tris[3,5-bis(ethoxymethyl)pyrazolyl]methane, HC[3,5-(CH₃CH₂OCH₂)₂pz]₃ (compound 9) 3,5-bis(ethoxymethyl)pyrazole

This compound has been obtained using the same methodologies described in Example 3a for 3,5-bis(methoxymethyl)pyrazole. Yield: 55% (529 mg, 2.87 mmol).

¹H NMR (CDCl₃, δ ppm): 8.39 (1H, br, NH), 6.20 (1H, s, H-4 (pz)), 4.49 (4H, s, CH₂), 3.50 (4H, q, 6.9 Hz, CH₂), 1.17 (6H, t, 6.9 Hz, CH₃). ¹³C NMR (CDCl₃, δ ppm): 145.5 (C-3/5 (pz)), 103.7 (C-4 (pz)), 65.7 (CH₂), 64.8 (CH₂), 15.0 (CH₃).

tris[3,5-bis(ethoxymethyl)pyrazolyl]methane, HC [3,5-(CH₃CH₂OCH₂)₂pz]₃ (compound 9)

Compound 9 has been prepared using the same methodology described for compound 7. Starting from 3,5-bis(ethoxymethyl)pyrazolyl (485 mg, 2.63 mmol) a slightly yellow oil was obtained, after the appropriate work-up. Yield: 47% (230 mg, 0.41 mmol).

¹H NMR (CDCl₃, δ ppm): 8.68 (1H, s, CH), 6.32 (3H, s, H-4 (pz)), 4.41 (6H, s, CH ₂), 4.32 (6H, s, CH ₂) 3.46 (6H, q, 6.9 Hz, CH ₂) 3.34 (6H, q, 6.9 Hz, CH ₂), 1.16 (9H, t, 6.9 Hz, CH₃), 1.06 (9H, t, 6.9 Hz, CH₃). ¹³C NMR (CDCl₃, δ ppm): 150.0 (C-3/5(pz)), 141.7 (C-3/5(pz)), 107.2 (C-4(pz)), 79.0 (C—H), 66.4 (CH₂), 65.8 (CH₂), 65.5 (CH₂), 62.9 (CH₂), 15.1, (CH₃), 14.9 (CH₃). FTICR/MS(+) (m/z): 563.4 [M+H]⁺ (46%).

Example 4 Production of a chelator of formula I having alkoxy groups at the 3-, 4- and 5-positions of the pyrazolyl ring: tris[3,4,5-tris(methoxymethyl)pyrazolyl]methane, HC[3,4,5-(CH₃OCH₂)₃pz]₃ (compound 10) (FIG. 5). Trimethyl-3,4,5-pyrazoletricarboxylate

3,4,5-Pyrazoletricarboxylic acid (D. Chambers, W. A. Denny, J. Org. Chem., 1985, 50, 4736-4738) (2.15 g, 10.7 mmol) was dissolved in methanol (80 mL) and concentrated H₂SO₄ (0.85 mL) was added. After overnight reflux, the solvent was evaporated in the rotary evaporator. The residue was dissolved in water (80 mL) and the product extracted with CHCl₃ (4×80 mL). The organic phase was dried over MgSO4, separated and the solvent evaporated under vacuum. The crude product was purified by silica-gel chromatography using gradient elution, from 100% CHCl₃ to 100% MeOH. The collected fractions were evaporated under vacuum and the white solid analysed. Yield: 32% (830 mg, mmol).

¹H NMR (CDCl₃, δ ppm): 3.95 (3H, s, COOCH ₃), 3.93 (6H, s, COOCH ₃).

Trimethyl 1-trityl-3,4,5-pyrazoletricarboxylate

The synthesis of this compound was done by tritylation of trimethyl-3,4,5-pyrazoletricarboxylate (1680 mg, 6.94 mmol) using a procedure similar to the one described in examples 2 and 3 for the synthesis of other tritylated pyrazole derivatives. The starting pyrazole reacted with sodium hydride (405 mg, 10.13 mmol) in dry dimethylformamide (80 mL) for 30 min at room temperature. Then, trityl chloride (1935 mg, 6.94 mmol) was added and the reaction mixture stirred overnight at room temperature. After removal of the solvent under vacuum, the resulting residue was washed with distilled water, n-hexane, and finally dried under vacuum to afford the title compound, as a white microcrystalline solid. Yield: 90% (3.034 g, 6.26 mmol).

¹H NMR (CDCl₃, δ ppm): 7.30-7.24 (9H, m, Ph), 7.13-7.09 (6H, m, Ph), 3.84 (3H, s, COOCH ₃), 3.80 (3H, s, COOCH ₃), 3.19 (3H, s, COOCH ₃).

1-trityl-3,4,5-tris(hydroxymethyl)pyrazole

To a solution of trimethyl 1-trityl-3,4,5-pyrazoletricarboxylate (3.034 g, 6.26 mmol) in dry THF (60 mL) were added 50 mL of 1.0 M LiAlH₄ in Et₂O. The resulting solution was stirred for about 16 h at room temperature (20-25° C.). After this time, the reaction was quenched by the slow addition of 1.5 mL of distilled water, followed by addition of 1.5 mL of 10% NaOH and 3.5 mL of water. The solids formed were removed by filtration, and 80 mL of dichloromethane was added to the filtrate. The organic phase was separated, washed with distilled water (70 mL) and, after separation from the aqueous phase, was dried over MgSO4. After separation of the organic phase, the solvent was evaporated under vacuum yielding a yellowish solid. Yield: 73% (1.823 g, 4.54 mmol).

¹H NMR (CDCl₃, δ ppm): 7.29 (9H, m, Ph), 7.08 (6H, m, Ph), 4.67 (2H, d, CH ₂), 4.63 (2H, d, CH ₂), 4.05 (2H, d, CH ₂), 2.68 (1H, tr, OH), 2.41 (1H, tr, OH), 0.84 (1H, tr, OH).

1-trityl-3,4,5-tris(methoxymethyl)pyrazole

To a solution of 1-trityl-3,4,5-tris(hydroxymethyl)-pyrazole (1.823 g, 4.54 mmol) in dry THF (60 mL) was added 685 mg of a 60% NaH suspension (28.5 mmol), and the mixture was stirred for 4 h at room temperature (20-25° C.). After this time, 4.35 mL of methyl iodide (mmol) was added and the mixture was allowed to react for 2 h. The solvent was removed under vacuum and the residue redissolved in dichloromethane (100 mL) and the organic phase was washed with distilled water. The organic phase was separated, dried over MgSO4 and the filtrate was vacuum dried. The white microcrystalline solid was formulated as the above mentioned compound based on NMR analysis. Yield: 98% (2.12 g, mmol).

¹H NMR (CDCl₃, δ ppm): 7.26 (9H, m, Ph), 7.12 (6H, m, Ph), 4.44 (4H, d, CH ₂), 3.78 (2H, s, CH ₂), 3.32 (3H, s, CH ₃), 3.31 (3H, s, CH ₃), 2.77 (3H, s, CH ₃).

3,4,5-tris(methoxymethyl)pyrazole

This compound is a white microcrystalline solid obtained by deprotection of 1-trityl-3,4,5-tris(methoxymethyl)pyrazole (2.125 g, 4.80 mmol), as described in example 2b for 4-methoxymethylpyrazole. Yield: 78% (752 mg, 3.75 mmol).

¹H NMR (CDCl₃, δ ppm): 10.85 (1H, br, NH), 4.51 (4H, s, CH₂), 4.37 (2H, s, CH ₂), 3.35 (6H, s, CH ₃) 3.29 (3H, s, CH ₃). ¹³C NMR (CDCl₃, δ ppm): 144.1 (C-3/5 (pz)), 113.7 (C-4 (pz)), 65.5 (CH₂), 63.7 (CH₂), 58.1 (CH₃), 57.7 (CH₃).

tris[3,4,5-(methoxymethyl)pyrazolyl]methane, HC [3,4,5-(CH₃OCH₂)₃pz]₃ (compound 10)

To a solution of 3,4,5-tris(methoxymethyl)pyrazole (202 mg, 1.01 mmol) and [NBu₄]Br (16.8 mg, 1.01 mmol) in distilled water (1.5 mL) was slowly added Na₂CO₃ (660 mg, mmol). Then, 0.8 mL of CHCl₃ was added and the mixture was heated gently under reflux for 5 days. The organic phase was separated, washed with water and dried over MgSO₄. Compound was purified by silica-gel flash chromatography using methanol/dichloromethane (2:98) as eluent. Yield: 34% (116 mg, 0.24 mmol).

¹H NMR (CDCl₃, δ ppm): 8.83 (1H, s, CH), 4.42 (6H, s, CH ₂), 4.38 (6H, s, CH ₂), 4.37 (6H, s, CH ₂) 3.25 (18H, s, CH ₃) 3.13 (9H, s, CH ₃).). ¹³C NMR (CDCl₃, δ ppm): 148.5 (C-3/5 (pz)), 139.6 (C-3/5 (pz)), 117.9 (C-4 (pz)), 78.7 (CH), 67.1 (CH₂), 63.6 (CH₂), 57.8 (CH₃), 57.5 (CH₃).

Example 5 Production of a Chelator of Class II Having an Alkoxy Group at the Primary Amine Example 5a N-(2-methoxyethyl)-2,2-di(1H-pyrazol-1-yl)ethanamine: MeO(CH₂)₂NHCH₂CH(pz)₂ (compound 11) (FIG. 6)

To a solution of 2,2′-bis(pyrazolyl)ethanamine (327 mg, 1.8 mmol) in dry ethanol (15 mL) was added excess of 1-chloro-2-methoxyethane (526 μL; 5.8 mmol), K₂CO₃ (1.281 g, 9.2 mmol) and KI (30 mg, 0.18 mmol), and the mixture refluxed for 4 days. After this time, the solvent was removed under vacuum and the residue was applied on a silica gel column which was eluted with MeOH/CHCl₃ (5:95). Removal of the solvent from the collected fractions yielded the title compound in the form of a brown oil. Yield: 21% (89 mg, 0.38 mmol).

¹H NMR (CDCl₃): δ_(H) 7.58 (d, H-3/5 (pz), 2H), 7.53 (d, H-3/5 (pz), 2H), 6.51 (t, CH, 1H), 6.25 (t, H-4 (pz), 2H), 3.69 (d, —NHCH ₂CH, 2H), 3.41 (t, OCH ₂, 2H), 3.28 (s, —OCH, 3H), 2.27 (t, NCH ₂CH₂, 2H). ¹³C NMR (CDCl₃): δ_(C) 140.3 (C-3 (pz)), 128.9 (C-5 (pz)), 106.6 (C-4 (pz)), 75.0 (CH), 71.8 (CH₂), 58.8 (CH₃O), 51.6 (CH₂), 48.7 (CH₂). FT/ICR-MS (+) (m/z): 236.2 [M+H]⁺ (10%).

Example 6 Production of a Chelator of Class III Having Ether Groups at the Secondary Amine and/or at the 3- and 5-Position of the Pyrazolyl Rings Example 6a bis(2-(3,5-bis(methoxymethyl)-1H-pyrazol-1-yl)ethyl)amine: {3,5-(CH₃OCH₂)₂}pz(CH₂)₂NH(CH₂)_(2{)3,5-(CH₂OCH₃)₂ pz)} (compound 12) (FIG. 7)

A suspension of NaH (0.240 g, 10 mmol) in dry DMF (20 mL) was slowly added to 3,5-bis(methoxymethyl)pyrazole (500 mg; 3.42 mmol) in dry DMF (10 mL). The reaction mixture was left at room temperature for 4 h. A solution of bis-(2-chloroethyl)amine hydrochloride (305 mg, 1.71 mmol) was then slowly added, and the mixture reacted at room temperature for 5 days. The solvent was evaporated and the crude compound was purified by silica gel chromatography (eluent: CHCl₃/MeOH (95:5)). The collected fractions were vacuum dried affording compound 12 as slightly yellow oil Yield: 40% (26.1 mg; 0.68 mmol).

¹H NMR (300 MHz, CDCl₃, δ (ppm)): 6.18 (s, H-4 (pz), 2H), 4.39 (d, —CH₂, 8H), 4.14 (t, —CH₂, 4H), 3.35 (s, —OCH₃, 6H), 3.28 (s, —OCH₃, 6H), 3.00 (t, —CH₂, 4H); ¹³C NMR (75.37 MHz, CDCl₃, δ (ppm)) 148.3, 139.4, 106.1, 68., 64.3, 57.9, 57.6, 49.0, 48.9.

Example 6b 2-(3,5-bis(methoxymethyl)-1H-pyrazol-1-yl)-N-(2-(3,5-bis(methoxymethyl)-1H-pyrazol-1-yl)ethyl)-N-(2-methoxyethyl)ethanamine: {3,5-(CH₃OCH₂)₂}pz(CH₂)₂N((CH₂)₂OMe)(CH₂)_(2{)3,5-(CH₂OCH₃)₂pz)} (compound 13) (FIG. 7) Ethyl 2-(bis(2-(3,5-bis(methoxymethyl)-1H-pyrazol-1-yl)ethyl)amino)acetate

To a solution of compound 12 (0.261 g, 0.68 mmol) in acetonitrile (15 mL) was added potassium carbonate (193 mg, 1.4 mmol) and potassium iodide (6 mg, 0.03.4 m mol). To this suspension was then added ethyl bromo acetate (155 μL, 1.4 mmol). The reaction mixture was refluxed overnight, under nitrogen. The supernatant was separated and the solvent evaporated under vacuum. The orange solid obtained was analysed. Yield: (280 mg; 0.6 mmol).

¹H NMR (300 MHz, CDCl₃, δ (ppm)): 6.15 (s, H-4 (pz), 2H), 4.39 (d, —CH₂, 8H), 4.13-4.06 (q, —CH₂, 8H), 4.01 (t, —CH₂, 4H), 3.37-3.28 (m, —OCH₃+—CH₂, 12+2H), 3.07 (t, —CH₂, 4H), 1.21 (t, —CH₃, 3H).

2-(bis(2-(3,5-bis(methoxymethyl)-1H-pyrazol-1-yl)ethyl)amino)ethanol

This compound has been obtained by reacting a solution of ethyl 2-(bis(2-(3,5-bis(methoxymethyl)-1H-pyrazol-1-yl)ethyl)amino)acetate (280 mg; 0.6 mmol) in dry THF (10 mL) with LiAlH₄. The title compound was isolated following the same methodology above described for the synthesis of other (hydroxymethyl)pyrazoles (see example 6). Yield: 60% (170 mg, 0.4 mmol).

¹H NMR (300 MHz, CDCl₃, δ (ppm)): 6.18 (s, H-4 (pz), 2H), 4.38-4.34 (m, —CH₂, 4H), 3.99 (t, —CH₂, 4H), 3.49 (m, —CH₂, 2H), 3.34 (s, —OCH₃, 6H), 3.30 (s, —OCH₃, 6H), 2.92 (t, —CH₂, 4H), 2.67 (t, —CH₂, 2H).

{3,5-(CH₃OCH₂)₂}pz(CH₂)₂N((CH₂)₂OMe)(CH₂)_(2{)3,5-(CH₂OCH₃)₂pz)} (compound 13)

Compound 13 has been obtained by reacting a solution of 2-(bis(2-(3,5-bis(methoxymethyl)-1H-pyrazol-1-yl)ethyl)amino)ethanol (170 mg, 0.4 mmol) in dry THF (10 mL) with NaH and CH₃I. Yield: 30% (46 mg; 0.12 mmol).

¹H NMR (CDCl₃): δ_(H) 6.17 (s, H-4 (pz), 2H, 4.40-4.34 (m, —CH₂, 8H), 4.07-3.95 (m, —CH₂, 4H), 3.35-3.23 (m, —OCH₃+—CH₂, 15+2H), 3.21-2.89 (m, —CH₂, 4H), 2.73-2.69 (m, —CH₂, 2H).

Example 7 Production of Tricarbonyl Rhenium Complexes with Chelators of General Formulae (I), (II) or (III)

Although the preferred complexes according to this invention, in that they are useful for diagnosis, are the ^(99m)Tc complexes, the Re complexes are used as a model to characterise them, as the ^(99m)Tc complexes are not available in sufficient quantities to be characterised by the normal analytical techniques, such as NMR. After the Re complexes have been thus characterised, HPLC is performed on both the Re complexes and the corresponding ^(99m)Tc complexes to determine if the retention time is the same for both.

The synthesis of the Re complexes was done by reaction of the chelators of general formula (I), (II) or (III) with common Re(I) starting materials, such as (NEt₄)₂[Re(CO)₃Br₃] or [Re(CO)₃(H₂O)₃]Br (FIG. 8). In all the complexes the chelators act as neutral and tridentate, as confirmed by ¹H and ¹³C NMR spectroscopy (FIGS. 9 and 10) and by X-ray diffraction analysis (FIG. 11).

Example 7a

Tricarbonyl Rhenium Complex of O-methyl-1,1,1-tris(pyrazolyl)ethanol (compound 1): [Re(CO)₃{CH₃OCH₂C(pz)₃}] (compound 14)

A solution of (NEt₄)₂[Re(CO)₃Br₃] (80 mg, 0.104 mmol) and compound 1 (27 mg, 0.104 mmol) in ethanol (15 mL) was heated under reflux for about 16 h. The solvent was removed under vacuum and the residue extracted with THF. Compound 14 was recovered as a beige solid, after removal of THF, washing with toluene and drying under vacuum. Yield: 71% (45 mg, 0.074 mmol).

Analysis calculated for C₁₅H₁₄N₆O₄BrRe: C, 29.61%; H, 2.32%; N, 13.81%. Found: C, 28.27%; H, 2.48%; N, 11.23%. IR Data (KBr, v/cm⁻¹): 1914 (vs) and 2042 (s) (C≡O).

¹H NMR (CDCl₃, δ_(H) ppm): 8.98 (1H, br, H-3/5 (pz)), 8.49 (2H, br, H-3/5 (pz)), 8.01 (3H, br, H-3/5 (pz)), 6.58 (3H, br, H-4 (pz)), 6.12 (2H, s, CH ₂), 4.06 (3H, s, CH ₃). ¹³C NMR (CDCl₃, δ_(C) ppm): 193.8 (br, CO), 147.2 (C-3/5 (pz)), 135.8 (C-3/5 (pz)), 134.6 (C-3/5 (pz)), 109.9 (C-4 (pz)), 109.0 (C-4 (pz)), 85.2 (Cpz₃), 70.4 (CH₂), 60.7 (CH₃).

Example 7b Tricarbonyl Rhenium Complex of O— ethyl-1,1,1-tris(pyrazolyl)ethanol (compound 2): [Re(CO)₃{CH₃CH₂OCH₂C(pz)₃}] (compound 15)

Complex 15 was synthesized as above described for compound 14 by reaction of (NEt₄)₂[Re(CO)₃Br₃] with CH₃CH₂OCH₂C(pz)₃ (2). However, compound 15 was always obtained slightly contaminated with [NEt₄]Br, due to their similar solubilities in the most common solvents.

IR Data (KBr, v/cm⁻¹): 1926 (s), 1946 (s) and 2042 (s) (CO).

¹H NMR (CDCl₃, δ ppm): 9.22 (1H, br, H-3/5 (pz)), 8.49 (2H, d, H-3/5 (pz)), 8.02 (2H, br, H-3/5 (pz)), 7.98 (1H, br, H-3/5 (pz)), 6.58 (3H, tr, H-4 (pz)), 6.27 (2H, s, CH ₂), 4.38 (2H, q, CH ₂), 1.37 (3H, tr, CH ₃).

Example 7c Tricarbonyl Rhenium Complex of O-n-propyl-1,1,1-tris(pyrazolyl)ethanol (compound 3): [Re(CO)₃{CH₃CH₂CH₂OCH₂C(pz)₃}] (compound 16)

A solution of (NEt₄)₂[Re(CO)₃Br₃] (100 mg, 0.13 mmol) and compound 3 (40 mg, 0.14 mmol) in ethanol (15 mL) was heated under reflux for about 16 h. The solvent was evaporated under vacuum and the residue was washed with THF and water. The insoluble solid was dried under vacuum and formulated as compound 16. Yield: 30% (25 mg, 0.039 mmol).

Analysis calculated for C₁₇H₁₈N₆O₄BrRe: C, 32.08%; H, 2.85%; N, 13.20%. Found: C, 31.39%; H, 2.41%; N, 12.72%. IR Data (KBr, v/cm⁻¹): 1940 (vs) and 2042 (s) (C≡O).

¹H NMR (CDCl₃, δ ppm): 9.20 (1H, br, H-3/5 (pz)), 8.39 (2H, br, H-3/5 (pz)), 8.04 (2H, br, H-3/5 (pz)), 7.99 (1H, br, H-3/5 (pz)), 6.58 (3H, br, H-4 (pz)), 6.25 (2H, br, CH ₂), 4.27 (2H, br, CH ₂), 1.75 (2H, br, CH ₂), 0.98 (3H, br, CH ₃).

Example 7d Tricarbonyl Rhenium Complex of tris[3,5-(methoxymethyl)pyrazolyl]methane (compound 7)

[Re(CO)₃{HC[3,5-(CH₃OCH₂)₂pz]₃}] (compound 17)

Complex 17 was obtained by reacting (NEt₄)₂[Re(CO)₃(H₂O)₃]Br with HC[3,5-(CH₃OCH₂)₂pz]₃ (compound 7) in ethanol under reflux for about 16 h. IR and ¹H NMR analyses of the crude product, after removal of the solvent under vacuum, have shown the formation of the desired compound.

IR Data (KBr, v/cm⁻¹): 1945 (vs) and 2037 (s) (C≡O).

¹H NMR (CDCl₃, δ ppm): 9.47 (1H, s, CH), 6.70 (3H, s, H-4 (pz)), 4.96 (6H, s, CH ₂), 4.63 (6H, s, CH ₂) 3.55 (9H, s, CH ₃) 3.50 (9H, s, CH ₃).

Example 8 Production of Tricarbonyl ^(99m)Tc Complexes with Chelators of General Formula (I) (FIG. 12)

In a nitrogen-purged glass vial, 100 μL of a 10⁻³10⁻² M aqueous solution of compounds 1-3, 7, 10 and of the already described ligands HC(pz)₃ and HC(3,5-Me₂pz)₃ were added to 900 μL (5-15 mCi) of the organometallic precursor fac-[^(99m)Tc(OH₂)₃(CO)₃]⁺, and the mixture was heated at 70-100° C. for 30-60 min. After this time, complexes 14a-18a, 23a and 24a have been obtained in >90% yield, as checked by gradient HPLC analysis (100% aqueous 0.1% CF₃COOH solution→100% CH₃CN (or 100% CH₃OH)) using a Nucleosil column (10 μm, 250 mm×4 mm) The chemical identity of the ^(99m)Tc complexes was confirmed by comparing their HPLC chromatograms with the HPLC profile of the analogue Re complexes (FIG. 13). The characterization of the ^(99m)Tc complexes comprised also the determination of the octanol-water partition coefficient (log P_(o/w)) values by the multiple back extraction method under physiological conditions (n-octanol/0.1 M PBS, pH 7.4). The retention time and log P_(o/w) values obtained for complexes 14a-18a, 23a and 24a are given in Table 1.

TABLE 1 HPLC retention time (t_(R)) and log P_(o/w) values for complexes 6a-11a Complex t_(R)(min) log P_(o/w) 14a 20.6 0.32 15a 21.7 0.68 16a 20.8 1.18 17a 20.1 0.58 18a 20.8 0.64 23a 19.6 1.2 24a 16.6 0.55

Example 9 Biodistribution of the ^(99m)Tc complexes with Chelators of General Formula (I)

These studies have been designed as a preliminary screening tool to evaluate the biological profile of the cationic tricarbonyl complexes. The biodistribution of the complexes was evaluated in groups of 5 female CD-1 mice (randomly bred, Charles River) weighing approximately 20-25 g each and have been performed according to EEC Legislation (National Law 129/92) on ethical and animal care Animals were intravenously injected with 100 μl (1.5-8.0 MBq) of each preparation via the tail vein and were maintained on normal diet ad libitum. Mice were killed by cervical dislocation at 1 h and 2 h p.i. The injected radioactive dose and the radioactivity remaining in the animal after sacrifice were measured in a dose calibrator (Aloka, Curiemeter IGC-3, Tokyo, Japan). The difference between the radioactivity in the injected and sacrificed animal was assumed to be due to total excretion from whole body animal Blood samples were taken by cardiac puncture at sacrifice. Tissue samples of the main organs were then removed, weighted and counted in a gamma counter (Berthold). Biodistribution results were expressed as percentage of the injected dose per organ (% ID/organ) and/or per gram tissue (% ID/g). For blood, total activity was calculated assuming that this organ constitutes 6% of the total weight, respectively. The remaining activity in the carcass was also measured in a dose calibrator. Biodistribution data for some ^(99m)Tc complexes are shown in Tables 2 and 3. Table 4 also shows a direct comparison of biodistribution and relevant ratios for complexes 17a and 18a and the heart imaging agent ^(99m)Tc-Sestamibi in the same animal model (% ID/g of wet organ).

TABLE 2 Biodistribution data for complexes 14a-18a, 23a, 24a (% ID/g of wet organ). (23a) (24a) (14a) (15a) Organ 1 h 2 h 1 h 2 h 1 h 2 h 1 h 2 h Blood 1.4 ± 0.1 1.3 ± 0.2 5.4 ± 1.2 1.6 ± 0.3 1.5 ± 0.1 0.8 ± 0.1 0.5 ± 0.2 0.3 ± 0.1 Liver 18.7 ± 4.7  19.1 ± 5.3  9.9 ± 2.7 4.9 ± 1.3 6.9 ± 2.3 6.2 ± 2.1 3.8 ± 2.0 1.6 ± 0.6 Heart 4.1 ± 0.7 4.3 ± 1.8 1.9 ± 0.3 1.4 ± 0.3 1.3 ± 0.3 0.9 ± 0.2 3.6 ± 0.5 1.8 ± 0.7 Lung 3.2 ± 0.3 2.1 ± 0.7 1.4 ± 0.3 1.2 ± 0.2 0.8 ± 0.2 0.8 ± 0.3 0.9 ± 0.1 0.6 ± 0.3 Kidney 44.7 ± 5.1  40.0 ± 5.2  7.2 ± 0.5 3.9 ± 0.8 3.3 ± 0.9 2.7 ± 0.4 5.0 ± 1.4 2.8 ± 0.5 (16a) (17a) (18a) Organ 1 h 2 h 1 h 2 h 1 h 2 h Blood 0.36 ± 0.06 0.22 ± 0.06 0.9 ± 0.1 0.9 ± 0.2 0.52 ± 0.09 0.34 ± 0.02 Liver 4.3 ± 1.6 2.1 ± 0.5 8.7 ± 1.8 5.3 ± 0.9 5.1 ± 0.9 5.0 ± 2.4 Heart 1.8 ± 0.4 0.4 ± 0.2 10.1 ± 3.1  9.9 ± 3.1 18.2 ± 3.2  15.8 ± 2.1  Lung 0.9 ± 0.5 0.29 ± 0.04 3.1 ± 0.8 2.3 ± 0.8 4.3 ± 1.3 2.2 ± 0.3 Kidney 8.6 ± 2.1 7.8 ± 3.0 25.0 ± 3.3  14.7 ± 1.8  16.1 ± 2.3  9.9 ± 0.9

TABLE 3 Heart/Blood, Heart/Liver and Heart/Lung ratios for complexes 14a-18a, 23a and 24a. (23a) (24a) (14a) (15a) Ratios 1 h 2 h 1 h 2 h 1 h 2 h 1 h 2 h Heart/Blood 2.6 ± 0.3 3.8 ± 0.1 0.34 ± 0.06 0.9 ± 0.2 0.9 ± 0.1 1.1 ± 0.1 8.2 ± 2.9 9.6 ± 0.6 Heart/Liver 0.22 ± 0.03 0.25 ± 0.07 0.20 ± 0.04 0.29 ± 0.05 0.18 ± 0.03 0.3 ± 0.1 1.4 ± 0.9 1.3 ± 0.7 Heart/Lung 1.3 ± 0.1 2.0 ± 0.3 1.3 ± 0.3 1.2 ± 0.3 1.7 ± 0.3 1.6 ± 0.4 4.0 ± 0.7 4.3 ± 1.4 (16a) (17a) (18a) Ratios 1 h 2 h 1 h 2 h 1 h 2 h Heart/Blood 4.6 ± 0.9 1.6 ± 0.5 11.2 ± 3.5  11.5 ± 2.2  35.0 ± 5.3  47.3 ± 2.3  Heart/Liver 0.5 ± 0.2 0.15 ± 0.05 1.1 ± 0.2 1.6 ± 0.5 3.5 ± 0.9 3.5 ± 0.9 Heart/Lung 2.2 ± 0.8 3.3 ± 0.4 4.6 ± 1.8 4.9 ± 1.1 7.0 ± 0.6

TABLE 4 Biodistribution data and relevant ratios for complexes 17a and 18a and the heart imaging agent ^(99m)Tc-Sestamibi (% ID/g of wet organ). (17a) (18a) ^(99m)Tc-Sestamibi 5 min 60 min 120 min 5 min 60 min 120 min 5 min 60 min 120 min Organ Blood 0.9 ± 0.1 0.9 ± 0.2 1.14 ± 0.09 0.52 ± 0.09 0.34 ± 0.02 2.0 ± 0.4 1.2 ± 0.4 1.1 ± 0.3 Liver 8.7 ± 1.8 5.3 ± 0.9 9.2 ± 0.9 5.1 ± 0.9 5.0 ± 2.4 9.0 ± 1.8 5.6 ± 0.3 5.0 ± 2.1 Heart 10.1 ± 3.1  9.9 ± 3.1 15.8 ± 1.7  18.2 ± 3.2  15.8 ± 2.1  9.4 ± 0.4 8.0 ± 0.6 7.9 ± 1.0 Lung 3.1 ± 0.8 2.3 ± 0.8 4.2 ± 0.5 4.3 ± 1.3 2.2 ± 0.3 2.4 ± 0.6 1.3 ± 0.2 0.9 ± 0.2 Kidney 25.0 ± 3.3  14.7 ± 1.8  43.4 ± 4.3  16.1 ± 2.3  9.9 ± 0.9 34.2 ± 2.4  14.8 ± 6.1  9.7 ± 1.8 Ratios Heart/Blood 11.2 ± 3.5  11.5 ± 2.2  13.9 ± 1.2  35.0 ± 5.3  47.3 ± 2.3  4.1 ± 0.7 7.9 ± 3.6 7.4 ± 2.3 Heart/Liver 1.1 ± 0.2 1.6 ± 0.5 1.7 ± 0.3 3.5 ± 0.9 3.5 ± 0.9 0.9 ± 0.2 1.4 ± 0.1 1.8 ± 0.7 Heart/Lung 3.3 ± 0.4 4.6 ± 1.8 3.8 ± 0.4 4.9 ± 1.1 7.0 ± 0.6 3.5 ± 0.8 6.3 ± 0.6 9.2 ± 1.1 

What is claimed is:
 1. A compound comprising Formula (I), (II), or (III):

wherein, n is an integer selected from 1 or 2, and at least one of R₁, R₂, R₃, and R₄ is a linear group of the type —R^(x)—O—R^(Y) or a macrocyclic ether group, and each of the other three R groups is independently hydrogen; a linear or branched, saturated or unsaturated C₁ to C₉ alkyl; a saturated or unsaturated carbocyclic group; a saturated or unsaturated heterocyclic or heteroaliphatic group with one or more atoms selected from O, N and S, wherein said carbocyclics, heterocyclics and heteroaliphatics are optionally substituted by one or more linear or branched, saturated or unsaturated C₁ to C₉ alkyls; an ether group —R^(x)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), wherein R^(x) and R^(Y) are independently linear or branched, saturated or unsaturated C₁ to C₉ alkyl, or saturated or unsaturated carbocyclics, any of which alkyl and/or carbocyclic groups may be substituted or unsubstituted, and provided that when the tridentate chelator is of the type of formula (I), when R₁ is —R^(x)—O—R^(Y) wherein R^(Y) is substituted, and R₂, R₃, and R₄ are each hydrogen, R^(Y) is not substituted apically by a further tris(pyrazolyl)methane moiety; and when R₁ is —R^(x)—O—R^(Y) and R₂, R₃ and R₄ are each hydrogen, R₁ cannot be —CH₂—O—CH₂— (p-^(t)Bu-C₆H₄).
 2. The compound of claim 1, wherein the compound is complexed with a ^(99m)Tc radioisotope.
 3. A compound comprising Formula (I):

wherein, at least one of R₁, R₂, R₃, and R₄ is a linear or macrocyclic ether group of the type —R^(x)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), respectively, and each of the other three R groups is independently hydrogen; a linear or branched, saturated or unsaturated C₁ to C₉ alkyl; a saturated or unsaturated carbocyclic group; a saturated or unsaturated heterocyclic or heteroaliphatic group with one or more atoms selected from O, N and S, wherein said carbocyclics, heterocyclics and heteroaliphatics are optionally substituted by one or more linear or branched, saturated or unsaturated C₁ to C₉ alkyls; an ether group —R^(x)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), wherein R^(x) and R^(Y) are independently linear or branched, saturated or unsaturated C₁ to C₉ alkyl, or saturated or unsaturated carbocyclics, any of which alkyl and/or carbocyclic groups may be substituted or unsubstituted, provided that when R₁ is —R^(x)—O—R^(Y) wherein R^(Y) is substituted, and R₂, R₃, and R₄ are each H, R^(Y) is not substituted apically by a further tris(pyrazolyl)methane moiety; and when R₁ is —R^(x)—O—R^(Y) and R₂, R₃ and R₄ are each H, R₁ cannot be —CH₂—O—CH₂— (p-^(t)Bu-C₆H₄).
 4. The composition of claim 3, wherein R1 is chosen from CH₂OCH₃, CH₂OCH₂CH₃, and CH₂OCH₂CH₂CH₃.
 5. The composition of claim 3, wherein R2, R3, and R4 are hydrogen.
 6. The composition of claim 3, wherein R1 is hydrogen; R2 and R4 are C₁ to C₃ alkyl; and R3 is the ether group —R^(x)—O—R^(Y), where R^(x) and R^(Y) are linear C₁ to C₉ alkyl.
 7. The composition of claim 6, wherein R2 and R4 are methyl, and R3 is CH₂CH₂OCH₃.
 8. The composition of claim 3, wherein R1 and R3 are hydrogen, and R2 and R4 are the ether group —R^(x)—O—R^(Y), where R^(x) and R^(Y) are linear C₁ to C₉ alkyl.
 9. The composition of claim 8, wherein R2 and R4 are CH₂OCH₃.
 10. The composition of claim 3, wherein R1 is hydrogen and R2, R3, and R4 are the ether group —R^(x)—O—R^(Y), where R^(x) and R^(Y) are linear C₁ to C₉ alkyl.
 11. The composition of claim 10, wherein R2, R3, and R4 are CH₂OCH₃.
 12. The composition of claim 3, wherein R1, R2, and R4 are hydrogen and R3 is the ether group —R^(x)—O—R^(Y), where R^(x) and R^(Y) are linear C₁ to C₉ alkyl.
 13. The composition of claim 12, wherein R3 is CH₂OCH₃.
 14. The composition of claim 12, wherein R3 is CH₂OCH₂CH₃.
 15. The composition of claim 3, wherein R2, R3, and R4 are hydrogen.
 16. A compound comprising Formula (II):

wherein, n is an integer selected from 1 or 2, and at least one of R₁, R₂, R₃, and R₄ is a linear or macrocyclic ether group of the type —R^(x)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), respectively, and each of the other three R groups is independently hydrogen; a linear or branched, saturated or unsaturated C₁ to C₉ alkyl; a saturated or unsaturated carbocyclic group; a saturated or unsaturated heterocyclic or heteroaliphatic group with one or more atoms selected from O, N and S, wherein said carbocyclics, heterocyclics and heteroaliphatics are optionally substituted by one or more linear or branched, saturated or unsaturated C₁ to C₉ alkyls; an ether group —R^(x)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), wherein R^(x) and R^(Y) are independently linear or branched, saturated or unsaturated C₁ to C₉ alkyl, or saturated or unsaturated carbocyclics, any of which alkyl and/or carbocyclic groups may be substituted or unsubstituted.
 17. The composition of claim 16, wherein n is equal to 1; R1 is the ether group —R^(x)—O—R^(Y), where R^(x) and R^(Y) are linear C₁ to C₉ alkyl; and R2, R3, and R4 are hydrogen.
 18. The composition of claim 17, wherein R1 is CH₂CH₂OCH₃.
 19. A compound comprising Formula (III):

wherein, n is an integer selected from 1 or 2, and at least one of R₁, R₂, R₃, and R₄ is a linear or macrocyclic ether group of the type —R^(x)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), respectively, and each of the other three R groups is independently hydrogen; a linear or branched, saturated or unsaturated C₁ to C₉ alkyl; a saturated or unsaturated carbocyclic group; a saturated or unsaturated heterocyclic or heteroaliphatic group with one or more atoms selected from O, N and S, wherein said carbocyclics, heterocyclics and heteroaliphatics are optionally substituted by one or more linear or branched, saturated or unsaturated C₁ to C₉ alkyls; an ether group —R^(x)—O—R^(Y) or [(CH₂)_(x)O]_(y)(CH₂)_(z) (x=2-3, y=3-8, z=2-5), wherein R^(x) and R^(Y) are independently linear or branched, saturated or unsaturated C₁ to C₉ alkyl, or saturated or unsaturated carbocyclics, any of which alkyl and/or carbocyclic groups may be substituted or unsubstituted.
 20. The composition of claim 19, wherein n is equal to 2; R1, R2, and R4 are the ether group —R^(x)—O—R^(Y), where R^(x) and R^(Y) are linear C₁ to C₉ alkyl; and R3 is hydrogen.
 21. The composition of claim 20, wherein R1 is CH₂CH₂OCH₃, and R2 and R4 are CH₂OCH₃. 